01289nam a2200265 a 450000100080000000500110000800800410001902400350006010000160009524501370011126001390024830000110038752004160039865000160081465000210083065000220085165000150087365000160088865000220090465000140092665000180094065000290095870000160098770000200100321141172020-02-13 2019 bl uuuu u00u1 u #d7 a10.1094/PHYTO-109-10-S2.12DOI1 aYOSHIDA, F. aA rapid method for detection of Sclerotinia sclerotiorum sensitivity to Fluazinam and Carbendazim fungicides.h[electronic resource] aIn: PLANT HEALTH - APS ANNUAL MEETING, 2019, Cleveland. Abstrats of presentations. Cleveland: American Phytopathological Societyc2019 aS.282. aThe objective of this work was to develop a method to screen S. sclerotiorum resistance to fungicides by employing 96-well microplates and Alamar Blue dye, used as a cell viability indicator. Experimental procedures involved 20 isolates of S. sclerotiorum from different Brazilian regions, tested at 1 × 102, 103 and 104 ascospores mL?1, along with Carbendazim and Fluazinam fungicides at 10, 100 and 1000 ppm. aCarbendazim aChemical control aDisease detection aFungicides aSclerotinia aControle Químico aFungicida aMancha Branca aSclerotinia Sclerotiorum1 aBARBOSA, E.1 aLOBO JUNIOR, M.