02780naa a2200337 a 450000100080000000500110000800800410001902200140006002400380007410000190011224501690013126000090030052018070030965000170211665000200213365000200215365000100217365000180218365000090220165000130221065000100222365000220223365000100225565000170226565000140228265000220229670000200231870000220233870000250236077300570238521140152019-11-07       2020    bl uuuu     u00u1 u    #d  a0944-50137 a10.1016/j.micres.2019.1263532DOI1 aCHAIBUB, A. A.  aMolecular and morphological characterization of rice phylloplane fungi and determination of the antagonistic activity against rice pathogens.h[electronic resource]  c2020  aCladosporium spp. is a cosmopolitan fungal genus. In the literature, it has been reported as a biological agent for controlling several plant diseases, but its mechanism of action has never been clarified. The present study aims to identify Cladosporium spp. based on the DNA phylogeny of nine isolates obtained from the phylloplane of rice and their potential antagonistic activity against the main fungal pathogens that affect rice crop. Nine isolates of Cladosporium spp. were identified based on DNA phylogeny, molecular and morphological characterization, and their antagonistic effects with the rice pathogens C. miyabeanus, M. oryzae, M. albescens and S. oryzae. Four isolates were selected to study lytic enzymes such as β-1,3-glucanase, chitinase and protease, and only one isolate was selected for a conidial germination and appressoria formation assay. The nine isolates were identified as C. cladosporioides, C. tenuissimum and C. subuliforme. Four isolates, identified as C. cladosporioides, inhibited the mycelial growth of rice pathogens such as C1H (68.59%) of S. oryzae, C5 G (74.32%) of C. miyabeanus, C11 G (75.97%) of M. oryzae and C24 G (77.39%) of M. albescens. C24 G showed a high activity of lytic enzymes, was tested against C. miyabeanus and M. oryzae, and inhibited conidial germination and appressorium formation by more than 59.36%. The characterization of C. cladosporioides suggested this species as a potential bioagent for the management of several rice diseases, especially rice blast. This is the first time that a potential biological agent from the genus Cladosporium identified at the species level was isolated from the rice phylloplane, and some of its mechanisms of action were demonstrated, such as increasing lytic enzyme activity against rice pathogens.  aCladosporium  aDrug antagonism  aEnzyme activity  aFungi  aMycoparasites  aRice  aTaxonomy  aArroz  aDoença de Planta  aFungo  aOryza Sativa  aPatógeno  aTaxonomia Vegetal1 aSOUSA, T. P. de1 aARAÚJO, L. G. de1 aFILIPPI, M. C. C. de  tMicrobiological Researchgv. 231, 126353, Jan. 2020.