01757naa a2200229 a 450000100080000000500110000800800410001902400520006010000170011224501430012926000090027252010280028165000170130965300430132665300140136970000150138370000150139870000210141370000190143470000190145377300550147221107532019-11-19       2019    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.jare.2019.04.0072DOI1 aHARTMANN, A.  aAssessment of the structural and functional diversities of plant microbiotabachievements and challenges: a review.h[electronic resource]  c2019  aAnalyses of the spatial localization and the functions of bacteria in host plant habitats through in situ identification by immunological and molecular genetic techniques combined with high resolving microscopic tools and 3D-image analysis contributed substantially to a better understanding of the functional interplay of the microbiota in plants. Among the molecular genetic methods, 16S-rRNA genes were of central importance to reconstruct the phylogeny of newly isolated bacteria and to localize them in situ. However, they usually do not allow resolution for phylogenetic affiliations below genus level. Especially, the separation of opportunistic human pathogens from plant beneficial strains, currently allocated to the same species, needs genome-based resolving techniques. Whole bacterial genome sequences allow to discriminate phylogenetically closely related strains. In addition, complete genome sequences enable strain-specific monitoring for biotechnologically relevant strains. In this mini-review we present  aAzospirillum  aDiazotrophic plant beneficial bacteria  aHolobiont1 aFISHER, D.1 aKINZEL, L.1 aCHOWDHURY, S. P.1 aBALDANI, J. I.1 aROTHBALLER, M.  tJournal of Advanced Researchgv. 9, p. 3-13, 2019.