02556naa a2200313 a 450000100080000000500110000800800410001902200140006010000200007424501360009426000090023049000140023952016170025365000370187065000110190765000240191865000180194265000210196065000190198165000170200065000250201770000200204270000220206270000250208470000250210970000230213470000280215777300570218521098482019-12-06       2019    bl uuuu     u00u1 u    #d  a1916-97521 aSANTIAGO, R. T.  aObtaining triploid hybrids by means of open pollinations and controlled crosses involving diploids parental.h[electronic resource]  c2019  a0021-8596  aThe creation of triploid hybrids is an important genetic improvement strategy for the development of new commercial citrus scion varieties. The objective of this work was to quantify the frequency of triploids obtained from natural and controlled crosses of different mandarin varieties under varying environmental conditions in the state of Bahia. The experiments were conducted in the municipalities of Cruz das Almas (Recôncavo Baiano region) and Mucugê (Chapada Diamantina region). The first experiment was based on fruits from open pollinations of the varieties ?Page?, ?Ortanique?, ?Ellendale?, ?Clemenules?, ?Swatow?, ?Piemonte?, ?Fortune?, ?South Africa?, ?Montenegrina?, ?Kincy?, ?Span Americana?, ?Fremont, ?Nova?, ?Dancy? and ?Murcott?, and the second involved fruits from controlled crosses of female parents ?Nova?, ?Fortune? and ?Ortanique? and male parents ?Page?, ?Montenegrina?, ?Swatow?, ?Fremont? and ?Kincy?. The seeds selected were inoculated in test tubes containing approximately 10 mL of Woody Plant Medium (WPM). When the plants reached circa 60 days of age, leaf samples were removed for quantification of the DNA by the flow cytometry technique. In Mucugê, three triploids were identified from open pollinations, ?Clemenules? (1) and ?Ortanique? (2), while in Cruz das Almas, no triploids were obtained. In the controlled crosses, triploids were only obtained in Mucugê: ?Ortanique? × ?Montenegrina? (4), ?Ortanique? × ?Kincy? (1) and ?Ortanique? × ?Swatow? (2). Based on the data, it is suggested that the environmental conditions of Mucugê favored the formation of triploids.  aBreeding and Genetic Improvement  aCitrus  aGenetic improvement  aHybridization  aIn vitro culture  aFruta Cítrica  aHibridação  aMelhoramento Vegetal1 aSOUZA, A. da S.1 aLEDO, C. A. da S.1 aALMEIDA, L. A. da H.1 aSANTOS, K. C. F. dos1 aGESTEIRA, A. da S.1 aSOARES FILHO, W. dos S.  tJournal of Agricultural Sciencegv.. 11, n. 5, 2019.