02814naa a2200301 a 450000100080000000500110000800800410001902400430006010000220010324502550012526000090038052017690038965000240215865000170218265000200219965000130221965000100223265300220224265300210226465300340228570000200231970000220233970000170236170000230237870000220240170000270242377300620245021071792025-01-10       2019    bl uuuu     u00u1 u    #d7 a10.1016/j.anireprosci.2019.02.0042DOI1 aFONSECA, J. F. da  aCervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Inês ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin.h[electronic resource]  c2019  aThe effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Inês ewes (Days 6–7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 ug of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n=13) or without (PGF-OT; n=11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estroussynchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n=12) or estradiol cypionate (EC-PGF-OT; n=12) i.m. along with 37.5 ug of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P&gt0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 ± 1.1 embryos/ewe collected with mean duration of the flushing procedure being ˜36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P&gt0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being ˜33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P&lt0.05). The interval from EB administration to the beginning of transcervical penetration can affect the efficacy of embryo recovery procedures utilizing a combined EB/d-cloprostenol/oxytocin pre-treatment.  aAnimal reproduction  aCloprostenol  aEmbryo transfer  aOxytocin  aSheep  aCervical dilation  aEstradiol esters  aTranscervical embryo recovery1 aZAMBRINI, F. N.1 aGUIMARÃES, J. D.1 aSILVA, M. R.1 aOLIVEIRA, M. E. F.1 aBARTLEWSKI, P. M.1 aSOUZA-FABJAN, J. M. G.  tAnimal Reproduction Sciencegv. 203, p. 25-32, Apr. 2019.