02663naa a2200349 a 450000100080000000500110000800800410001902400390006010000250009924501030012426000090022752016590023665000190189565000180191465000170193265000320194965000200198165000120200165000100201365000210202365000210204465300280206565300300209365300230212370000230214670000240216970000190219370000210221270000180223370000200225177300420227121067082019-11-07       2019    bl uuuu     u00u1 u    #d7 a10.1016/j.toxicon.2019.02.0032DOI1 aANDRADE, I. R. A. de  aInactivation of lectins from castor cake by alternative chemical compounds.h[electronic resource]  c2019  aAbstract: Enabling the use of castor cake in animal feeding is an excellent alternative strategy to reduce feed costs. The cake is a by-product derived from the extraction of the castor oil by the biodiesel industry whose chemical composition is satisfactory despite the presence of antinutritional factors like toxic lectins, which require detoxification before it can be used as a dietary ingredient. The aim of the present study was to evaluate alternative chemical sources in the degradation and inactivation of ricin and Ricinus communis agglutinin (RCA), two lectins from castor cake. Ten chemical compounds were evaluated: sodium hydroxide, monodicalcium phosphate, dicalcium phosphate, calcium oxide, calcium hydroxide, calcitic limestone, magnesian limestone, urea, potassium chloride, and sodium chloride. Gel electrophoresis indicated 100% lectin degradation only in the cakes treated with 90 g sodium hydroxide and 2500 mL water per kg of cake. The hemagglutination assay was crucial to providing innocuousness to the treated cakes, with total absence of hemagglutinating activity observed in the castor cakes treated with 60 or 90 g sodium hydroxide in water volumes equal to or higher than 1500 mL/kg of castor cake and in the cakes treated with 90 g calcium oxide with 2500 or 3000 mL water/kg castor cake. Thus, though depending on the concentration of the chemical compound and on the volume of water per kilogram of treated cake, sodium hydroxide and calcium oxide showed to be promising chemical products for degradation and complete inactivation of the lectins present in castor cake to allow its use as an ingredient in animal diets.  aAnimal feeding  aCalcium oxide  aDenaturation  aDetoxification (processing)  aElectrophoresis  aLectins  aRicin  aSodium hydroxide  aRicinus Communis  aHaemagglutination tests  aHemagglutinating activity  aRicinus agglutinin1 aCÂNDIDO, M. J. D.1 aPOMPEU, R. C. F. F.1 aFEITOSA, T. S.1 aBOMFIM, M. A. D.1 aSALLES, H. O.1 aEGITO, A. S. do  tToxicongv. 160, p. 47-54, Feb. 2019.