02616naa a2200325 a 450000100080000000500110000800800410001902400380006010000200009824502000011826000090031852015610032765000190188865000230190765000140193065000270194465000190197165000210199065000330201165000240204465000220206865300150209065300190210570000260212470000240215070000200217470000180219470000190221277300590223121054102019-02-04       2018    bl uuuu     u00u1 u    #d7 a10.1016/j.micres.2018.08.0022DOI1 aMASCARIN, G. M.  aPhenotype responses to abiotic stresses, asexual reproduction and virulence among isolates of the entomopathogenic fungus Cordyceps javanica (HypocrealesbCordycipitaceae).h[electronic resource]  c2018  aSelecting entomopathogenic fungal isolates with resilience to environmental stresses, optimal mass production characteristics, and with high virulence to target pests favors the development of mycopesticides. A case in point, Cordyceps (= Isaria) javanica has been extensively investigated for non-chemical control of whiteflies worldwide. We phylogenetically characterized 11 native C. javanica isolates from Northeastern and Central Brazil. These isolates were screened for tolerance to heat-shock, UV-B radiation, osmotic and oxidative stresses, as well as conidial production on cereal grain and insecticidal activity against the whitefly Bemisia tabaci (MEAM 1) in the laboratory. All isolates were pathogenic to whiteflies and significant (3-fold) differences in median lethal concentration were observed among isolates. Furthermore, pronounced differences among isolates were found for stress factors and conidial production. Using principal component analysis, our results highlighted three major clusters formed by isolates (i) resistant to osmotic and oxidative stress, (ii) resilient to UV-B, and (iii) with high virulence, conidial production and heat tolerance. Overall, isolate CG1228 performed best based on multi-stress resistance, mass production and virulence attributes in the laboratory. This study highlights the importance of exploring natural variation in entomopathogenic fungi for selection of appropriate isolates for effective biocontrol of insect pests coupled with mass production characteristics and abiotic stress tolerances.  aAbiotic stress  aBiological control  aCordyceps  aEntomopathogenic fungi  aOsmotic stress  aOxidative stress  aPrincipal component analysis  aControle Biológico  aFungo Entomógeno  aHeat-shock  aUV-B tolerance1 aPEREIRA-JUNIOR, R. A.1 aFERNANDES, E. K. K.1 aQUINTELA, E. D.1 aDUNLAP, C. A.1 aARTHURS, S. P.  tMicrobiological Researchgv. 216, p. 12-22, Nov. 2018.