01885naa a2200229 a 450000100080000000500110000800800410001902400380006010000190009824501310011726000090024852012000025765000160145765000220147365000100149570000200150570000220152570000170154770000190156470000210158377300510160421042562019-01-15       2018    bl uuuu     u00u1 u    #d7 a10.1016/j.phytol.2018.01.0072DOI1 aLIMA, G. da S.  aMetabolic response of Aspergillus sydowii to OSMAC modulation produces acetylcholinesterase inhibitors.h[electronic resource]  c2018  aFungi are an extraordinary source of bioactive metabolites especially because their metabolic profile can be modulated according to the conditions under which they are grown, a strategy known as OSMAC. Aspergillus sydowii has been showing to be a fungal species with a series of interesting biosynthetic pathways, but production of acethylcholinesterase inhibitors was still not sufficiently explored from this fungal species. In this work, A. sydowii was grown in three different media aiming at awaking biosynthetic routes silent in the works already reported in the literature, in order to produce acethylcholinesterase inhibitors. Using sucrose (150g/L) and peptone (12g/L) as sources of carbon and nitrogen respectively, a very active extract was obtained (84.5 + - 2.2% of acethylcholinesterase inhibition). Metabolites 1-5 were isolated from this extract. These metabolites are described from A. sydowii by the first time, to the best of our knowledge. Compound 2 was the most active AChE inhibitor (93.25 + - 0.41%). The results show how small changes in culturing conditions can modulate the production of bioactive secondary metabolites that are silent in other cultivation conditions.  aAspergillus  aDiketopiperazines  aFungo1 aROCHA, A. M. da1 aSANTOS, G. F. dos1 aSILVA, A. F.1 aMARRIEL, I. E.1 aTAKAHASHI, J. A.  tPhytochemistry Lettersgv. 24, p. 39-45, 2018.