01727naa a2200241 a 450000100080000000500110000800800410001910000190006024501130007926000090019250001470020152008650034865000230121365000240123665300230126065300090128365300270129265300200131965300190133970000210135870000180137977300880139711102972023-03-30       2004    bl uuuu     u00u1 u    #d1 aQUEIROZ, P. R.  aSurvival in soil and detection of co-transformed Trichoderma harzianum by nested PCR.h[electronic resource]  c2004  aNotas Científicas. Título em português: Sobrevivência em solo e detecção de co-transformantes de Trichoderma harzianum por PCR ?nested?.  aThe objective of this work was to evaluate the survival of two Trichoderma harzianum co-transformants, TE 10 and TE 41, carrying genes for green fluorescent protein (egfp) and for resistance to benomyl, during four weeks in a contained soil microcosm. Selective culture media were used to detect viable fungal material, whose identity was confirmed by the observation of the fluorescent phenotype by direct epifluorence microscopy. PCR using two nested primer pairs specific to the egfp gene was also used to detect the transformed fungi. Although it was not possible to reliably detect the egfp gene directly from soil extracts, an enrichment step involving selective culture of soil samples in liquid medium prior to DNA extraction enabled the consistent detection of the T. harzianum co-transformants by nested PCR for the duration of the incubation period.  abiological control  aControle Biológico  abenomyl resistance  aegfp  aresistência a benomil  atransformação  atransformation1 aINGLIS, M. C. V.1 aINGLIS, P. W.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 39, n. 4, p.403-405, abr. 2004