02635naa a2200385 a 450000100080000000500110000800800410001902200460006002400370010610000210014324501290016426000090029352014240030265000330172665000160175965000180177565000330179365000270182665000220185365000140187565000190188965000220190865000120193065300240194265300440196665300460201065300210205670000170207770000280209470000160212270000190213870000210215770000190217877300520219721027872021-07-02       2018    bl uuuu     u00u1 u    #d  a1598-2254 (impresso) / 2093-9280 (online)7 a10.5423/PPJ.OA.01.2018.00142DOI1 aFERRAZ, H. G. M.  aXanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil.h[electronic resource]  c2018  aBacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker?s group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.  aBacterial diseases of plants  aLeaf blight  aNursery crops  aPlant diseases and disorders  aXanthomonas axonopodis  aDoença de Planta  aEucalipto  aEucalyptus spp  aMancha Bacteriana  aViveiro  aCultivos de viveros  aEnfermedades bacterianas de las plantas  aEnfermedades y desórdenes de las plantas  aMarchitez foliar1 aBADEL, J. L.1 aGUIMARÃES, L. M. da S.1 aREIS, B. P.1 aTÓTOLA, M. R.1 aGONCALVES, R. C.1 aALFENAS, A. C.  tPlant Pathologygv. 34, n. 4, p. 269-285, 2018.