02961naa a2200277 a 450000100080000000500110000800800410001902400340006010000200009424502160011426000090033030000100033952020540034965000110240365000250241465300240243965300210246365300320248470000170251670000180253370000150255170000220256670000200258870000180260877300570262621020572023-01-24       2018    bl uuuu     u00u1 u    #d7 a10.3389/fmicb.2018.016472DOI1 aRAMOS, A. F. O.  aTucumã oil shifted ruminal fermentation, reducing methane production and altering the microbiome but decreased substrate digestibility within a RUSITEC fed a mixed hay - concentrate diet.h[electronic resource]  c2018  a11 p.  aAbstract Tucumã oil is sourced from the fruit pulp of the tucumã tree and contains high concentrations of unsaturated fatty acids and carotenoids. Due to these properties it may have the potential to decrease enteric methane (CH4) from ruminants when included in the diet. The objective of this study was to determine the effect of oil mechanically extracted from the fruit pulp of tucumã on fermentation characteristics, CH4 production and the microbial community using the rumen stimulation technique. Treatments consisted of a control diet (forage:concentrate; 70:30), and tucumã oil included at 0.5 or 1.0% (v/v). Addition of tucumã oil linearly decreased (P &lt 0.01) dry matter disappearance. Total gas (mL/d) and carbon dioxide (CO2) production (mL/d, mL/g DM) were unaffected (P ≥ 0.36) to increasing addition of tucumã oil where 0.5% (v/v) of Tucumã oil numerically increased both variables. Acetate and butyrate percentages of total VFA were linearly decreased (P ≤ 0.01) and propionate and valerate percentages of total VFA were linearly increased (P &lt 0.01) by increasing concentrations of tucumã oil added to the substrate. The ratio of acetate to propionate was linearly decreased (P &lt 0.01) with increasing concentration of tucumã oil. Methane production (mL/d) was linearly decreased (P = 0.04) with increasing addition of tucumã oil to the substrate. Tucumã oil reduced the bacterial richness and diversity when included at 1.0% (v/v) in both solid- and liquid- associated microbes. The abundance of the genera Fibrobacter and Rikenellaceae RC9 gut group were decreased and Pyramidobacter, Megasphaera, Anaerovibrio, and Selenomonas were enriched by the addition of 1.0% tucumã oil. In conclusion, tucumã oil resulted in the favorable shift in fermentation products away from acetate toward propionate, decreasing the production of CH4 when tucumã oil was included at 1.0% (v/v), however, substrate digestibility was also inhibited. The rumen microbiota was also altered by the addition of tucumã oil.  aCattle  aTerminalia Amazônia  aOil supplementation  aRumen microbiome  aRumen stimulation technique1 aTERRY, S. A.1 aHOLMAN, D. B.1 aBREVES, G.1 aPEREIRA, L. G. R.1 aSILVA, A. G. M.1 aCHAVES, A. V.  tFrontiers in Microbiologygv. 9, article 1647, 2018.