01736nam a2200313 a 450000100080000000500110000800800410001910000170006024501010007726001390017850000160031752007400033365000100107365000290108365000330111265000100114565000410115565000120119665000230120865000120123165000280124370000280127170000190129970000220131870000260134070000170136670000200138370000190140321010392018-12-13       2018    bl uuuu     u00u1 u    #d1 aFELIX, G. P.  aHigh seed transmission rates of cowpea mild mottle virus in common bean,.h[electronic resource]  aIn: CONGRESSO BRASILEIRO DE VIROLOGIA, 29.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 13., 2018, Gramado. Resumos... Brasília, DF: SBVc2018  aPoster 390.  aCommon bean (Phaseolus vulgaris) is an important staple crop in Brazil and is affected by several viruses, including Cowpea mild mottle virus - CPMMV (genus Carlavirus, family Betaflexiviridae). CPMMV is transmitted by the whitefly Bemisia tabaci and has been found infecting beans in high incidences in Central and Northeastern regions of the country. African strains of CPMMV were reported to be seed-transmitted in cowpea, soybean and bean cultivars. For the Brazilian CPMMV from bean and soybean isolates seed transmission assays using visualization of symptoms and ELlSA as evaluation methods were negative. In this work, we used the highly sensitive qPCR and RT-PCR techniques to assess seed transmission of CPMMV in common bean.  aBeans  aCowpea mild mottle virus  aPlant diseases and disorders  aSeeds  aViral diseases of animals and humans  aFeijão  aPhaseolus Vulgaris  aSemente  aTransmissão de Doença1 aALVES-FREITAS, D. M. T.1 aLIMA, B. P. de1 aLACERDA, A. L. M.1 aCARVALHO, M. M. R. de1 aVIDAL, A. H.1 aFARIA, J. C. de1 aRIBEIRO, S. G.