02560naa a2200373 a 450000100080000000500110000800800410001902200230006002400350008310000200011824501710013826000090030952013540031865000230167265000220169565000350171765000140175265000150176665000100178165300390179165300380183065300150186870000200188370000220190370000200192570000250194570000250197070000250199570000210202070000190204170000150206070000280207577300830210321006652019-01-15 2018 bl uuuu u00u1 u #d a1573-0972 (Online)7 a10.1007/s11274-018-2568-72DOI1 aSILVA, J. F. da aScreening of plant growth promotion ability among bacteria isolated from field-grown sorghum under different managements in Brazilian drylands.h[electronic resource] c2018 aSorghum [Sorghum bicolor (L.) Moench] is a multipurpose grass cultivated in drylands due to its adaptation to drought. However the characteristics of sorghum-associated bacteria are not known in the Brazilian drylands. The aim of this study was to isolate and evaluate the plant growth promotion potential bacteria from field-grown sorghum under two irrigation and manure application levels in a Brazilian semi-arid reagion. Sorghum was irrigated with 3 or 1 mm day? 1 and fertilized or not with liquid goat manure. Bacteria were obtained from surface-disinfected roots applying two nitrogen-free semi-solid media. The bacteria were evaluated for the presence of nifH gene, 16S rRNA sequences, calcium-phosphate solubilization, production of auxins and siderophores and for sorghum growth promotion. We obtained 20 out of 24 positive bacteria for nifH. The isolates were classified as in six different genera. All isolates produced auxins “in vitro”, six bacteria produced siderophores and three Enterobacteriaceae solubilized calcium-phosphate. At least ten bacteria resulted in the increased total N content in the sorghum shoots, comparable to fertilization with 50 mg N plant?1 week? 1 and to inoculation with Azospirillum brasilense Ab-V5. Enterobacter sp. ESA 57 was the best sorghum plant-growth promoting bacteria isolated in this study. aEnterobacteriaceae aNitrogen fixation aSorghum bicolor subsp. bicolor aBactéria aInoculante aSorgo aFixação biológica de nitrogenio aPromoção do crescimento vegetal aSemiárido1 aSILVA, T. R. da1 aESCOBAR, I. E. C.1 aFRAIZ, A. C. R.1 aSANTOS, J. W. M. dos1 aNASCIMENTO, T. R. do1 aSANTOS, J. M. R. dos1 aPETERS, S. J. W.1 aMELO, R. F. de1 aSIGNOR, D.1 aFERNANDES JUNIOR, P. I. tWorld Journal of Microbiology and Biotechnologygv. 34, n. 12, 186, nov. 2018.