01692naa a2200277 a 450000100080000000500110000800800410001902200140006010000210007424501330009526000090022852008890023765000110112665000180113765000130115565000180116865000140118670000230120070000260122370000250124970000190127470000250129370000200131870000220133877300540136020995882018-11-19       2018    bl uuuu     u00u1 u    #d  a0168-16561 aSOARES, T. da C.  aValidating a probe from GhSERK1 gene for selection of cotton genotypes with somatic embryogenic capacity.h[electronic resource]  c2018  aSubstantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures. High expression of transcripts was found in embryogenic genotypes, and the results were confirmed by the RTPCR- blotting using a non-radioactive probe. The regeneration ability was confirmed in embryogenic genotypes. We confirmed that GhSERK1 can be used as marker for estimating the somatic embryogenesis ability of cotton plants.  aCotton  aEmbryogenesis  aAlgodão  aEmbriogénese  aGenótipo1 aSILVA, C. R. C. da1 aCARVALHO, J. M. F. C.1 aCAVALCANTI, J. J. V.1 aLIMA, L. M. de1 aMELO FILHO, P. de A.1 aSEVERINO, L. S.1 aSANTOS, R. C. dos  tJournal of Biotechnologygn. 270, p. 44-50, 2018.