02899naa a2200325 a 450000100080000000500110000800800410001902200140006002400430007410000250011724501460014226000090028852018940029765000230219165000150221465000200222965000270224965000310227665000160230765000170232365000140234065000190235465000240237365000350239765000150243270000200244770000200246770000190248777300670250620980362018-12-07       2018    bl uuuu     u00u1 u    #d  a1439-04187 ahttps://doi.org/10.1111/jen.125042DOI1 aSANTOS, T. T. M. dos  aEnhanced mortality of Bemisia tabaci nymphs by Isaria javanica combined with sublethal doses of chemical insecticides.h[electronic resource]  c2018  aThe aim of this current study was to evaluate the mortality of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) nymphs by the combination between the entomopathogenic fungus Isaria javanica (Friedrichs & Bally) Samson & Hywel-Jones and synthetic chemical insecticides. The bioefficacy of I. javanica and the insect growth regulators named spiromesifen and buprofezin was tested alone and in combination against B. tabaci nymphs under screenhouse conditions. The in vitro compatibility between these two control agents was previously assessed under laboratory conditions. The sublethal concentration (LC25) of these insecticides towards second-instar nymphs was determined and then mixed with the fungal treatments to investigate the type of interaction. All I. javanica isolates at 5 × 107 conidia/ml inflicted nymphal mortality by up to 62.4%. The insecticides did not influence the germination and mycelial growth of the selected I. javanica isolate CG1282. In general, the insecticide-fungus combinations increased nymphal mortalities in comparison with their single counterparts. Combinations with the fungus and buprofezin or spiromesifen augmented nymphal mortality by 10% and 24%, respectively, in comparison with the fungus alone. Additive interaction was found with the combination of the I. javanica CG1282 at 1 × 106 conidia/ml and spiromesifen at 1.56 ppm, and additive and synergistic interactions were achieved with the fungus at 5 × 106 conidia/ml and spiromesifen at 3.12, 6.25 and 12.5 ppm. Additive interactions were also observed from mixtures of fungus at 5 × 106 and buprofezin at 3.12 and 6.25 ppm. Only combinations of I. javanica with spiromesifen reduced to some extent the mycosis on dead nymphs. The joint action of I. javanica with sublethal doses of these insecticides may be a promising tool to assist in the integrated management system for B. tabaci.  aBiological control  aBuprofezin  aCordycipitaceae  aEntomopathogenic fungi  aIntegrated pest management  aPlant pests  aSpiromesifen  aSynergism  aBemisia Tabaci  aControle Biológico  aFungo Para Controle Biológico  aInseticida1 aQUINTELA, E. D.1 aMASCARIN, G. M.1 aSANTANA, M. V.  tJournal of Applied Entomologygv. 142, n. 6, p. 598-609, 2018.