02958naa a2200433 a 450000100080000000500110000800800410001910000170006024501450007726000090022250001470023152016400037865000110201865000160202965000110204565000170205665000180207365000160209165000160210765000280212365000150215165000110216665000140217765000280219165000170221965000150223665000150225165000130226665300160227965300120229570000190230770000170232670000170234370000160236070000200237670000190239670000180241577300910243320977422018-10-18       2018    bl uuuu     u00u1 u    #d1 aPORTO, Y. F.  aOccurrence of brucellosis, leptospirosis and neosporosis in cows with retained placenta in Southwest Paraná, Brazil.h[electronic resource]  c2018  aTítulo em português: Ocorrência de brucelose, leptospirose e neosporose em vacas com retenção de placenta no Sudoeste do Paraná, Brasil.  aThe aim of this study was to measure the occurrence of brucellosis, leptospirosis and neosporosis in cows from 25 family farms milk, with semi-intensive grazing system, located in the Southwest region of Paraná, Brazil. Eighty-four cows with retained placenta, between July 2013 to July 2014, diagnosed b y the presence of fetal membranes in the uterus up to 12 hours after partum, decrease of appetite and of milk production, as well increase of rectal temperature were included into the study. The animals were submitted to blood collection for evaluating the seropositivity for brucellosis, leptospirosis and neosporosis. The blood was collected at 15 to 45 days postpartum, avoiding false negative results due to immune deficiency observed in the transition period. After collection, the blood was centrifuged, the serum was packed in three aliquots, identified and subsequently frozen. Serology was performed to diagnose brucellosis (technique of buffered acidified antigen), leptospirosis (through the microscopic agglutination test - MAT) and neosporosis (indirect immunofluorescence test). Animals were considered positive when presenting titer &gt1:100 (leptospirosis) and 1:200 (neosporosis). No animal with placenta retention presented Brucella abortus bacteria; 39% (33/84) were reactive to one or more Leptospira serovars, 15% (13/84) were positive to Neospora caninum, and 4% (4/84) had both diagnosis, being reactive for leptospirosis and neosporosis. In conclusion, data from this experiment inspires greater attention to eptospirosis and neosporosis in dairy cattle presenting placenta retention in southwest Paraná.  aBrazil  aBrucellosis  aCattle  aDairy cattle  aLeptospirosis  aNeosporosis  aParasitoses  aReproductive efficiency  aBacteriose  aBovino  aBrucelose  aEficiência Reprodutiva  aLeptospirose  aNeosporose  aParasitose  aPlacenta  aBacterioses  aParaná1 aPINTO NETO, A.1 aBERNARDI, F.1 aPOSSA, M. G.1 aMOTA, M. F.1 aMARTINEZ, A. C.1 aMERLINI, L. S.1 aBERBER, R. C.  tPesquisa Veterinária Brasileira, Brasília, DFgv. 38, n 8, p. 1537-1542, agosto 2018