02005naa a2200265 a 450000100080000000500110000800800410001910000170006024500720007726000090014952012440015865000260140265000190142865000130144765000400146065000170150065000160151765000100153365300310154365300250157465300230159965300220162270000200164477300750166420960172018-09-19       1991    bl uuuu     u00u1 u    #d1 aJONES, A. R.  aMetabolism of fructose-1,6-bisphosphate by mature boar spermatozoa.  c1991  aAbstract: Mature epididymal boar spermatozoa converted glucose and fructose to carbon dioxide and lactate and maintained high concentrations of ATP. In the presence of (S)-alpha-chlorohydrin these processes were inhibited and there was an accumulation of fructose-1,6-bisphosphate and dihydroxyacetone phosphate. With fructose-1,6-bisphosphate as the substrate, the concentration of ATP was maintained, carbon dioxide was evolved and dihydroxyacetone phosphate accumulated. Cells pre-incubated with (S)-alpha-chlorohydrin did not maintain ATP levels, evolved less carbon dioxide and produced dihydroxyacetone phosphate. Assays of incubates in which fructose-1,6-bisphosphate was used as the substrate showed the presence of equilibrium quantities of fructose-6-phosphate and glucose-6-phosphate which were not detected when either fructose or glucose were used as substrates. [14C]Fructose and [14C]glucose were not produced from [14C]fructose-1,6-bisphosphate in spermatozoal incubates which had or had not been pre-incubated with (S)-alpha-chlorohydrin. Evidence is presented that a high concentration of fructose-1,6-bisphosphate leads to the formation of fructose-6-phosphate and glucose-6-phosphate but not of fructose and/or glucose.  aAdenosine diphosphate  aCarbon dioxide  aFructose  aGlucose-6-phosphate 1-dehydrogenase  aPharmacology  aSpermatozoa  aSwine  aDihydroxyacetone Phosphate  aFructosediphosphates  aFructosephosphates  aGlucosephosphates1 aMONTAGUE, M. D.  tReproduction, Fertility and Developmentgv. 3, n. 5, p. 609-613, 1991.