02340naa a2200325 a 450000100080000000500110000800800410001902400360006010000280009624501330012426000090025750000220026652013570028865000170164565000160166265000170167865000180169565000190171365000090173265300130174165300270175470000180178170000170179970000200181670000190183670000200185570000220187570000190189777300980191620934102019-04-05       2017    bl uuuu     u00u1 u    #d7 a10.5380/bceppa.v35i2.603082DOI1 aSANTOS, A. S. de O. dos  aMajor goat milk proteinbSeparation and characterization by "lab-on-a-chip" microfluidic electrophoresis.h[electronic resource]  c2017  aBoletim do CEPPA.  aThis work presents the electrophoretic profile of goat and cow milk samples and their mixtures using microfluidic and conventional electrophoresis. The microfluidic method allowed the separation of the major caseins from milk, excepting the goat kappa-casein. Besides, the major whey proteins were separated with perfect distinction of A and B beta-lactoglobulin variants. Comparing to SDS-PAGE, a variation in the molecular weight was observed in all milk proteins. However, A and B beta-lactoglobulin variants could not be isolated using SDS-PAGE. Although urea-PAGE did not show high resolution among whey proteins, y-, k-, beta-, and alpha-caseins were clearly identified. This method also showed a lower limit detection of cow milk in mixture samples than the "lab-on-a-chip" electrophoresis. In both methods, the highest linearity obtained from plotting total percentage against cow milk concentration was observed by using cow ?s1-casein (R2 = 0.986 and R² = 0.973). This result indicates that microfluidic electrophoresis is an effective tool to detect the presence of some proteins in goat and cow milk, and in mixtures. Microfluidic chip technology might will complement the current methods for analyzing milk proteins, highlighting its speed amount of reagents and whey protein separation, which showed a better result than urea or SDS-PAGE  aAlpha-casein  aBeta-casein  aKappa-casein  aMilk proteins  aPolyacrylamide  aUrea  aCow milk  aProtein quantification1 aMEURER, V. M.1 aCOSTA, F. F.1 aPAIVA, I. M. de1 aFOGAÇA, G. N.1 aEGITO, A. S. do1 aFURTADO, M. A. M.1 aMARTINS, M. F.  tBoletim do Centro Pesquisa Processamento de Alimentos, Curitibagv. 35, n. 2, jul./dez. 2017.