02628naa a2200289 a 450000100080000000500110000800800410001910000170006024501370007726000090021450001560022352016260037965000100200565000190201565000150203465000210204965300090207065300330207970000180211270000260213070000160215670000180217270000190219070000160220970000230222577300900224820926282018-06-20       2018    bl uuuu     u00u1 u    #d1 aLOPES, E. S.  aMolecular diagnosis of diarrheagenic Escherichia coli isolated from Psittaciformes of illegal wildlife trade.h[electronic resource]  c2018  aTítulo em português: Diagnóstico molecular de Escherichia coli diarreiogênicas isoladas de Psittaciformes do tráfico ilegal de animais silvestres.  aDiarrheagenic Escherichia coli (DEC) are considered one of the major causes of human diarrhea in developing countries. Some studies have pointed wild birds as important reservoirs for these pathogens. However, scarce species from the Psittaciformes order have been investigated. This study aimed to evaluate the presence of DEC strains in Psittaciformes from illegal wildlife trade. A total of 78 E. coli strains isolated from cloacal swab samples of 167 Psittaciformes in the Ceará State, Brazil, were evaluated regarding the presence of the following DEC virulence genes by polymerase chain reaction (PCR): eaeA and bfpA genes (Enteropathogenic E. coli ? EPEC); stx1 and stx2 (Shiga toxin-producing E. coli - STEC); estA and eltB (Enterotoxigenic E. coli - ETEC); ipaH (Enteroinvasive E. coli - EIEC); aatA and aaiC (Enteroaggregative E. coli - EAEC). Positive strains for eaeA and bfpA genes were considered typical EPEC, while strain positive exclusively for the eaeA gene were classified as atypical EPEC. The eaeA gene was identified in 20 E. coli strains and bfpA in 22 isolates. In addition, 11 and 9 belonged to tEPEC and aEPEC, respectively. No strain was positive for stx1 or stx2. A total of 47 (60.3%) strains and a total of 136 birds (81.4%) were negative for the remaining DEC pathotypes investigated. In conclusion, psittacine from illegal wildlife trade in Ceará State, Brazil, presented a relevant prevalence of typical and atypical EPEC, potentially playing a role as reservoirs of DEC strains in the environment. Thus, proper  control measures must be adopted to block the spread of these pathogens.  aBirds  aPsittaciformes  aBacteriose  aEscherichia Coli  aEPEC  aTráfico de animal silvestre1 aMACIEL, W. C.1 aMEDEIROS, P. H. Q. S.1 aBONA, M. D.1 aBINDÁ, A. H.1 aLIMA, S. V. G.1 aGAIO, F. C.1 aTEIXEIRA, R. S. C.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 38, n. 4, p. 762-766, abril 2018