03011naa a2200433 a 450000100080000000500110000800800410001910000200006024501340008026000090021450001400022352016390036365000230200265000160202565000100204165000250205165000160207665000130209265000320210565000110213765000100214865000120215865000210217065000150219165000170220665300240222365300330224765300140228065300280229465300280232270000170235070000200236770000170238770000190240470000220242370000200244570000210246577300910248620918822018-05-23       2018    bl uuuu     u00u1 u    #d1 aMESQUITA, L. P.  aPlacental lesions associated with abortion and stillbirth in goats naturally infected by Neospora caninum.h[electronic resource]  c2018  aTítulo em português: Lesões placentárias associadas a abortos e natimortos em cabras naturalmente infectadas pelo Neospora caninum.  aNeospora caninum has been described as a parasite that sporadically causes reproductive problems in goats. Several aspects of the pathogenesis of neosporosis in naturally infected goats remain to be established. The aims of the present study were to characterize the placental lesions in goats naturally infected by N. caninum and to evaluate several diagnostic techniques for effective detection of this protozoan in the goat placenta. Some placentas in this study originated from abortion and stillbirth in which there were severe lesions. The lesions were characterized mainly by necrosis involving the mesenchyme of the chorionic villi and trophoblast cells often alongside mononuclear inflammation and in some cases with neutrophilic infiltration. N. caninum DNA was detected in these placentas, but parasite structures were not visualized through immunohistochemistry (IHC). However, five of 11 placentas from N. caninum-infected goats that gave birth to healthy kids had histological lesions characterized by mononuclear inflammation. Of these 11 placentas, N. caninum DNA was detected in seven, and N. caninum tachyzoites were detected in only one of these seven placentas using IHC. The present study demonstrates that severe lesions in the placenta are associated with abortion and stillbirth in caprine neosporosis and the placental alterations are likely involved in abortion pathogenesis. Moreover, the results highlight the importance of using more than one diagnostic technique for the detection of the protozoan in placentas because N. caninum cannot be reliably detected by histological and immunohistochemical tests.  aAbortion (animals)  aFetal death  aGoats  aImmunohistochemistry  aNeosporosis  aProtozoa  aTransplacental transmission  aAborto  aCabra  aCaprino  aNeospora Caninum  aNeosporose  aProtozoário  aImuno-histoquímica  aInfecção transplacentária  aNatimorto  aNecrotizing placentitis  aPlacentite necrotizante1 aCOSTA, R. C.1 aNOGUEIRA, C. I.1 aABREU, C. C.1 aORLANDO, D. R.1 aASCARI JUNIOR, I.1 aPECONICK, A. P.1 aVARASCHIN, M. S.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 38, n. 3, p. 444-449, março 2018