01900naa a2200217 a 450000100080000000500110000800800410001902200140006002400540007410000130012824501270014126000090026852012040027765300330148165300410151465300200155570000190157570000180159470000200161277300500163220911622018-05-04       2018    bl uuuu     u00u1 u    #d  a0929-13937 ahttps://doi.org/10.1016/j.apsoil.2018.03.0032DOI1 aOSEI, O.  aBacteria related to Bradyrhizobium yuanmingense from Ghana are effective groundnut micro-symbionts.h[electronic resource]  c2018  awere obtained, 30 of which were eventually found to nodulate groundnut plants. After testing the symbiotic potential of these isolates on groundnut on sterilized substrate, seven of them, designated as KNUST 1001?1007, were evaluated in an open field pot experiment using 15N-labelled soil. Although 15N dilution analyses did not indicate differences among treatments in the proportion of nitrogen (N) derived from the atmosphere (%Ndfa), all seven strains increased total N derived from N2 fixation by inoculated groundnut plants as compared to the non-inoculated control. Inoculation with KNUST 1002 led to total N accumulation as high as that of the groundnut reference strain 32H1. Genetic characterisation of the isolates by sequence analysis of 16S rRNA gene, 16S ? 23S rRNA intergenic transcribed spacer (ITS) region and nodC gene revealed that isolates KNUST 1003 and 1007 were related to Rhizobium tropici, a common bean symbiont. The other five isolates, including KNUST 1002 belonged to the Bradyrhizobium genus, being closely related to Bradyrhizobium yuanmingense. Therefore, this study revealed novel native Ghanaian rhizobia with potential for the development of groundnut inoculants.  aBiological nitrogen fixation  aGenetic diversity Arachis hypogaea L  aNative isolates1 aABAIDOO, R. C.1 aBODDEY, R. M.1 aROUWS, L. F. M.  tApplied Soil Ecologygv. 127, p. 41-50, 2018.