02548naa a2200277 a 450000100080000000500110000800800410001902400510006010000200011124501180013126000090024952017200025865000170197865000140199565300280200965300150203765300240205270000170207670000210209370000240211470000180213870000160215670000180217270000190219077300610220920900722018-04-05       2018    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s10482-017-0983-72DOI1 aSILVA, L. J. da  aRhodococcus psychrotolerans sp. nov., isolated from rhizosphere of Deschampsia antarctica.h[electronic resource]  c2018  aA novel actinobacterium, designated strain CMAA 1533T, was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533T was found to grow over a wide range of temperatures (4?28 °C) and pH (4?10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533T belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus. Sequence similarity calculations indicated that the novel strain is closely related to Rhodococcus degradans CCM 4446T, Rhodococcus erythropolis NBRC 15567T and Rhodococcus triatomae DSM 44892T (? 96.9%). The organism was found to contain meso-diaminopimelic acid, galactose and arabinose in whole cell hydrolysates. Its predominant isoprenologue was identified as MK-8(H2) and the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were identified as Summed feature (C16:1 ?6c and/or C16:1 ?7c), C16:0, C18:1 ?9c and 10-methyl C18:0. The G+C content of genomic DNA was determined to be 65.5 mol%. Unlike the closely related type strains, CMAA 1533T can grow at 4 °C but not at 37 °C and was able to utilise adonitol and galactose as sole carbon sources. Based on phylogenetic, chemotaxonomic and physiological data, it is concluded that strain CMAA 1533T (= NRRL B-65465T = DSM 104532T) represents a new species of the genus Rhodococcus, for which the name Rhodococcus psychrotolerans sp. nov. is proposed.  aActinomiceto  aRizosfera  aAntarctic actinomycetes  aCMAA 1533T  aPolyphasic approach1 aSOUZA, D. T.1 aGENUÁRIO, D. B.1 aVARGAS-HOYOS, H. A.1 aSANTOS, S. N.1 aROSA, L. H.1 aZUCCHI, T. D.1 aMELO, I. S. de  tAntonie van Leeuwenhoekgv. 111, n. 4, p. 629-636, 2018.