00927naa a2200157 a 450000100080000000500110000800800410001910000170006024501320007726000090020952004160021865000170063465300190065170000180067077300810068820891912025-06-26 1996 bl uuuu u00u1 u #d1 aSINGH, L. P. aEffect of ultra low temperature on acrosomal integrity of buck spermatozoa in tris and citrate dilutors.h[electronic resource] c1996 a50 ejaculates were obtained from 5 nondescript adult goats. The semen samples were diluted in egg yolk-fructose-citric acid-glycerol, with or without Tris, frozen in liquid nitrogen and stored for 24 h. The percentage of intact acrosomes declined from 88.76+or-0.43 before freezing to 68.90+or-0.82 in frozen-thawed semen in the Tris diluent, and from 85.98+or-0.47 to 56.70+or-1.15 in the diluent without Tris. aReproduction aSemen diluents1 aPURBEY, L. N. tThe Indian Journal of Animal Reproductiongv. 17, n. 1, p. 45-49, Jun. 1996.