00998naa a2200193 a 450000100080000000500110000800800410001910000130006024501610007326000090023452003870024365000100063065000110064065000170065170000240066870000210069270000170071377300740073020890922025-06-27       1994    bl uuuu     u00u1 u    #d1 aBASU, S.  aSeparation of acrosome of bull spermatozoa using detergents and assessment of its protein concentration by spectrophotometric method.h[electronic resource]  c1994  aSemen samples collected from 8 crossbred bulls were treated with detergent-0.75 mM cetyltrimethylammonium bromide (CTAB) or 0.025% Teepol xL-to separate the acrosome from the sperm head. CTAB was more effective than Teepol xL (separation in 98.125 vs. 75.875% of spermatozoa). Both detergents caused serious damage to the acrosome, which became shrunken, distorted or disintegrated.  aBulls  aCattle  aReproduction1 aBONDOPADHYAY, S. K.1 aROY CHOUDHRY, R.1 aGHOSH, P. R.  tIndian Journal of Animal Reproductiongv. 15, n. 2, p. 147-150, 1994.