01992naa a2200277 a 450000100080000000500110000800800410001910000130006024501240007326000090019752011570020665000170136365000240138065300160140465300200142070000250144070000250146570000260149070000220151670000160153870000210155470000190157570000260159470000220162077300720164220852772023-05-12       2017    bl uuuu     u00u1 u    #d1 aMUNK, M.  aUsing carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells.h[electronic resource]  c2017  aThe insertion of DNA into primary mammalian cells is an important step towards gene therapy and production of transgenic animals. Recently, carbon nanotubes have been explored as an efficient novel non-viral system for delivering genes to cells due to their unique structure and properties. However, their potential for transfecting primary bovine cells in non-toxic concentrations has not been tested. The purpose of this study was to evaluate the cytotoxicity of carboxylic acid-functionalized multiwalled carbon nanotubes (COOH-MWCNTs) and their use to deliver plasmid DNA encoding the gene of green fluorescent protein to bovine primary fibroblast cells. Flow-cytometry cell viability results have shown the non-toxic nature of COOH-MWCNTs at low concentrations. The frequency shifts in Raman spectroscopy showed that the plasmid DNA connects to the nanomaterial. Fluorescence imaging, flow-cytometry and PCR analysis confirmed that the COOH-MWCNT nanovectors delivered pDNA into primary fibroblast cells successfully. The results show that COOH-MWCNTs can be attractive alternatives for delivery of DNA into hard-to-transfect primary bovine cells.  acytotoxicity  agenetic improvement  aCOOH-MWCNTs  aDelivery of DNA1 aZANETTE, R. de S. S.1 aCAMARGO, L. S. de A.1 aSOUZA, N. L. G. D. de1 aALMEIDA, C. G. de1 aGERN, J. C.1 aGUIMARAES, A. S.1 aLADEIRA, L. O.1 aOLIVEIRA, L. F. C. de1 aBRANDAO, H. de M.  tBiomedical Physics & Engineering Expressgv. 3, n. 4, 045002, 2017.