02849naa a2200241 a 450000100080000000500110000800800410001902400480006010000240010824501890013226000090032152020220033065000130235265000090236565000190237465300120239365300190240570000250242470000180244970000180246770000280248577300940251320834322022-08-22       2017    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1590/1678-4162-93512DOI1 aCOIMBRA E SOUZA, V.  aCharacterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazilbthe need to identify to species level to avoid misidentification.h[electronic resource]  c2017  aABSTRACT - Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and coagulase-positive Staphylococcus aureus (2). Our results strongly support the need to identify the isolates to a species level in order to avoid misidentification and to be aware of the classification using the coagulase test alone. RESUMO - A mastite é uma inflamação da glândula mamária que afeta o gado leiteiro em todo o mundo, causando perdas econômicas. Staphylococcus coagulase-negativa (SCN) são a causa predominante desse tipo de infecção. Mostrou-se recentemente que Staphylococcus coagulase-positiva podem ser identificados erroneamente. Assim, o objetivo deste estudo foi caracterizar cepas de Staphylococcus spp. inicialmente classificados como Staphylococcus coagulase-negativa, isolados de búfalas com mastite subclínica. O leite de búfalas com mastite foi coletado, e nove cepas foram identificadas como SCN por testes fenotípicos. Metodologias moleculares identificaram as cepas como Staphylococcus chromogenes coagulase-negativa (5) Staphylococcus hyicus coagulase-positiva (2) e Staphylococcus aureus coagulase-positiva (2). Os resultados reforçam a necessidade de identificar as cepas em termos de espécie, a fim de se evitarem erros de identificação e estar atento à classificação utilizando o teste de coagulase sozinho.  amastitis  amilk  aStaphylococcus  aBuffalo  aCoagulase test1 aBRITO, M. A. V. P. e1 aCHAMON, R. C.1 aLAPORT, M. S.1 aGIAMBIAGI DE MARVAL, M.  tArquivo Brasileiro de Medicina Veterinária e Zootecniagv. 69, n. 6, p. 1669-1675, 2017.