02174naa a2200289 a 450000100080000000500110000800800410001902400530006010000300011324501250014326000090026852012790027765000210155665000280157765000140160565000390161965000110165865000290166965000140169865300130171270000180172570000180174370000230176170000170178470000190180177300640182020819652018-01-25       2017    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1007/s00203-017-1410-12DOI1 aVASCONCELLOS, R. L. de F.  aPseudomonas aestus sp. nov., a plant growth-promoting bacterium isolated from mangrove sediments.h[electronic resource]  c2017  aStrain CMAA ­ 1215T, a Gram-reaction-nega- tive, aerobic, catalase positive, polarly flagellated, motile, rod-shaped (0.5-0.8 × 1.3-1.9 -m) bacterium, was iso- lated from mangrove sediments, Cananéia Island, Brazil. Analysis of the 16S rRNA gene sequences showed that strain CMAA ­1215T forms a distinct phyletic line within the Pseudomonas putida subclade, being closely related to P. plecoglossicida ATCC 7 ­ 00383T, P. monteilii NBRC ­103158T, and P. taiwanensis BCRC ­17751T of sequence similarity of 98.86, 98.73, and 98.71%, respectively. Genomic comparisons of the strain CMAA 1215T ­ with its closest phylogenetic type strains using average nucleo- tide index (ANI) and DNA:DNA relatedness approaches revealed 84.3-85.3% and 56.0-63.0%, respectively. A mul- tilocus sequence analysis (MLSA) performed concatenating 16S rRNA, gyrB and rpoB gene sequences from the novel species was related with Pseudomonas putida subcluster and formed a new phylogenetic lineage. The phenotypic, physiological, biochemical, and genetic characteristics sup- port the assignment of CMAA ­1215T to the genus Pseu- domonas, representing a novel species. The name Pseu- domonas aestus sp.nov. is proposed, with CMAA 1 ­ 215T (=NRRL B-653100T = CBMAI ­1962T) as the type strain.  aGrowth promotion  aPlant growth substances  aBactéria  aEstimulante de crescimento vegetal  aMangue  aRegulador de crescimento  aSedimento  aMangrove1 aSANTOS, S. N.1 aZUCCHI, T. D.1 aSILVA, F. S. P. da1 aSOUZA, D. T.1 aMELO, I. S. de  tArchives of Microbiologygv. 199, n. 8, p. 1223-1229, 2017.