02128nam a2200397 a 450000100080000000500110000800800410001910000230006024501450008326001060022850001150033452008560044965000110130565000100131665000120132665000270133865000170136565000100138265000230139265000160141565000110143165000120144265000130145465000100146765000240147765000110150165300310151270000270154370000220157070000250159270000210161770000280163870000250166670000190169170000200171020808202019-09-23       2017    bl uuuu     u00u1 u    #d1 aOLIVEIRA, C. C. S.  aMini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium.h[electronic resource]  aRevista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 498, jan./mar. 2017.c2017  aEdição dos anais do XXII  Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.  aSperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen congelado/descongelado de ruminantes domésticos dispensa o uso da heparina em meio capacitante].  aCattle  aGoats  aHeparin  aIn vitro fertilization  aReproduction  aSheep  asperm capacitation  aSpermatozoa  aBovino  aCaprino  aHeparina  aOvino  aReprodução animal  aSêmen  aCapacitação espermática1 aSOUZA-FABJAN, J. M. G.1 aFONSECA, J. F. da1 aBATISTA, R. I. T. P.1 aBALARO, M. F. A.1 aSARAIVA, H. F. R. de A.1 aALFRADIQUE, V. A. P.1 aCÔRTES, L. R.1 aBRANDÃO, F. Z.