03050naa a2200493 a 450000100080000000500110000800800410001902400350006010000260009524501090012126000090023052016480023965000150188765000410190265000160194365000200195965000240197965000150200365000150201865000190203365000120205265000190206465000240208365000150210765000230212265000110214565000110215665300310216765300200219865300140221865300120223265300180224470000190226270000190228170000200230070000170232070000180233770000190235570000310237470000220240570000200242770000260244777300830247320783842023-10-31       2018    bl uuuu     u00u1 u    #d7 a10.1007/s11250-017-1398-12DOI1 aBEZERRA JUNIOR, R. Q.  aA panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection.  c2018  aAbstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE.  aBiomarkers  aCaprine arthritis encephalitis virus  aDairy goats  aGenetic markers  aInfectious diseases  aLentivirus  aProteomics  aViral proteins  aCaprino  aDoença animal  aGenética Molecular  aInfecção  aMarcador genético  aSêmen  aVírus  aArtrite Encefalite Caprina  aArylsulfatase A  aProteomic  aViroses  aVirus disease1 aELOY, A. M. X.1 aFURTADO, J. R.1 aPINHEIRO, R. R.1 aANDRIOLI, A.1 aMORENO, F. B.1 aLOBO, M. D. P.1 aMONTEIRO-MOREIRA, A. C. O.1 aMOREIRA, R. de A.1 aPINTO, T. M. F.1 aTEIXEIRA, M. F. da S.  tTropical Animal Health and Productiongv. 50, n. 1, n. 7, p. 43-48, Oct. 2018.