02023naa a2200409 a 450000100080000000500110000800800410001902400510006010000190011124501650013026000090029552008150030465000190111965000190113865000170115765000140117465000140118865000190120265000200122165000190124165000180126065000130127865300230129165300210131465300210133570000180135670000210137470000230139570000180141870000190143670000170145570000200147270000190149270000170151170000200152877300650154820733582017-12-06       2017    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1590/1519-6984.159152DOI1 aBOIJINK, C. L.  aAnesthesia by sprinkling method in the gills of tambaqui Colossoma macropomum does not influence intensity and morphology of monogeneans.h[electronic resource]  c2017  aThe present study evaluates the influence of anesthesia on the parasitic fauna of monogenea fish parasites, as its intensity and viability. Two experiments were conducted: Evaluation of an anesthetic method by sprinkling eugenol directly on gills and evaluation of monogenea motility and viability; Comparison of immersion and directly sprinkling on the gills with benzocaine and eugenol followed by evaluation on parasite intensity. The results suggest that the anesthetic sprinkling didn?t interfere in the parasite motility, morphology and body surface integrity analyzed by fluorescence method. The monogenean intensity in the gills was lower in fish anesthetized by immersion method compared to the sprinkling method and the control group. This method of anesthesia can be used in parasitological studies.  aAnimal welfare  ahistopathology  aParasitology  aslaughter  aBem-estar  aDoença animal  aEcologia animal  aHistopatologia  aParasitologia  aTambaqui  aAbate humanitário  aBem-estar animal  aHumane slaughter1 aMACIEL, P. O.1 aTAVARES-DIAS, M.1 aIWASHITA, M. K. P.1 aMORAIS, M. S.1 aHIDE, D. M. V.1 aSOUZA, N. C.1 aCOUTO, M. V. S.1 aMENESES, J. O.1 aCUNHA, F. S.1 aFUJIMOTO, R. Y.  tBrazilian Journal of Biologygv. 77, n. 2, p. 367-371, 2017.