03169naa a2200445 a 450000100080000000500110000800800410001910000170006024501680007726000090024550000570025452018830031165000130219465000170220765000110222465000090223565000160224465000180226065300130227865300220229165300250231370000170233870000200235570000170237570000150239270000230240770000160243070000220244670000170246870000170248570000190250270000250252170000230254670000190256970000190258870000230260770000170263070000230264777300530267020728072020-01-07       2017    bl uuuu     u00u1 u    #d1 aROSSE, I. C.  aWhole genome sequencing of Guzera cattle reveals genetic variants in candidate genes for production, disease resistance, and heat tolerance.h[electronic resource]  c2017  aNa publicação: Adhemar Zerlotini, Rui S. Verneque.  aAbstract In bovines, artificial selection has produced a large number of breeds which differ in production, environmental adaptation, and health characteristics. To investigate the genetic basis of these phenotypical differences, several bovine breeds have been sequenced. Millions of new SNVs were described at every new breed sequenced, suggesting that every breed should be sequenced. Guzerat or Guzera´ is an indicine breed resistant to drought and parasites that has been the base for some important breeds such as Brahman. Here, we describe the sequence of the Guzera´ genome and the in silico functional analyses of intragenic breed-specific variations. Matepaired libraries were generated using the ABI SOLiD system. Sequences were mapped to the Bos taurus reference genome (UMD 3.1) and 87% of the reference genome was covered at a 26X. Among the variants identified, 2,676,067 SNVs and 463,158 INDELs were homozygous, not found in any database searched, and may represent true differences between Guzera´ and B. taurus. Functional nalyses investigated with the NGS-SNP package focused on 1069 new, non-synonymous SNVs, splice-site variants (including acceptor and donor sites, and the conserved regions at both intron borders, referred to here as splice regions) and coding INDELs (NS/SS/I). These NS/SS/I map to 935 genes belonging to cell communication, environmental adaptation, signal transduction, sensory, and immune systems pathways. These pathways have been involved in phenotypes related to health, adaptation to the environment and behavior, and particularly, disease resistance and heat tolerance. Indeed, 105 of these genes are known QTLs for milk, meat and carcass, production, reproduction, and health traits. Therefore, in addition to describing new genetic variants, our approach provided groundwork for unraveling key candidate genes and mutations.  abreeding  adairy cattle  aGenome  aZebu  aBos Indicus  aGado de corte  aDiseases  aGenome sequencing  aSequência genética1 aASSIS, J. G.1 aOLIVEIRA, F. S.1 aLEITE, L. R.1 aARAUJO, F.1 aZERLOTINI NETO, A.1 aVOLPINI, A.1 aDOMINITINI, A. J.1 aLOPES, B. C.1 aARBEX, W. A.1 aMACHADO, M. A.1 aPEIXOTO, M. G. C. D.1 aVERNEQUE, R. da S.1 aMARTINS, M. F.1 aCOIMBRA, R. S.1 aSILVA, M. V. G. B.1 aOLIVEIRA, G.1 aCARVALHO, M. R. S.  tMammalian Genomegv. 28, n. 1-2, p. 66-80, 2017.