02057naa a2200277 a 450000100080000000500110000800800410001902400350006010000200009524501220011526000090023752012190024665300230146565300330148865300340152165300330155565300160158870000170160470000240162170000190164570000190166470000130168370000190169670000200171577300440173520708292017-06-12       2017    bl uuuu     u00u1 u    #d7 a10.1007/s00203-017-1398-62DOI1 aMATOS, G. F. de  aBradyrhizobium sacchari sp. nov., a legume nodulating bacterium isolated from sugarcane roots.h[electronic resource]  c2017  aMembers of the genus Bradyrhizobium are well-known as nitrogen-fixing microsymbionts of a wide variety of leguminous species, but they have also been found in different environments, notably as endophytes in non-legumes such as sugarcane. This study presents a detailed polyphasic characterization of four Bradyrhizobium strains (type strain BR 10280T), previously isolated from roots of sugarcane in Brazil. 16S rRNA sequence analysis, multilocus sequence analysis (MLSA) and analysis of the 16S-23S rRNA internal transcribed spacer showed that these strains form a novel clade close to, but different from B. huanghuaihaiense strain CCBAU 23303T. Average nucleotide identity (ANI) analyses confirmed that BR 10280T represents a novel species. Phylogenetic analysis based on nodC gene sequences also placed the strains close to CCBAU 23303T, but different from this latter strain, the sugarcane strains did not nodulate soybean, although they effectively nodulated Vigna unguiculata, Cajanus cajan and Macroptilium atropurpureum. Physiological traits are in agreement with the placement of the strains in the genus Bradyrhizobium as a novel species for which the name Bradyrhizobium sacchari sp. nov. is proposed.  aAverage nucleotide  aBiological nitrogen fixation  aFree Living nitrogen fixation  aMultilocus sequence analysis  aNon Legumes1 aZILLI, J. E.1 aARAUJO, J. L. S. de1 aLEME, M. M. P.1 aMELO, I. S. de1 aRADL, V.1 aBALDANI, J. I.1 aROUWS, L. F. M.  tArchives of Microbiology, 10 June 2017.