00996naa a2200217 a 450000100080000000500110000800800410001910000130006024501510007326000090022452003280023365000220056165300180058370000240060170000170062570000180064270000220066070000190068270000200070177300570072120703312017-12-04 2017 bl uuuu u00u1 u #d1 aOSEI, O. aPCR assay for direct specific detection of Bradyrhizobium elite strain BR 3262 in root nodule extracts of soil-grown cowpeah[electronic resource] c2017 aAims Successful inoculation of legume crops with rhizobia depends on dominating nodule occupancy with highly efficient strains. The aim of this study was to develop a rapid and reliable conventional PCR methodology to specifically detect an elite Bradyrhizobium strain in root nodule extracts from soil-grown cowpea plants. aVigna Unguiculata aReal time PCR1 aARAUJO, J. L. S. de1 aZILLI, J. E.1 aBODDEY, R. M.1 aAHIABOR, B. D. K.1 aABAIDOO, R. C.1 aROUWS, L. F. M. tPlant and Soilgv. 417, n. 1–2, p. 535–548, 2017