02510naa a2200385 a 450000100080000000500110000800800410001902400350006010000310009524501200012626000090024652013930025565000210164865000130166965000200168265000210170265000230172365300250174665300180177165300180178965300160180770000210182370000170184470000230186170000170188470000190190170000280192070000200194870000220196870000220199070000170201270000190202970000280204877300480207620701952017-08-31       2017    bl uuuu     u00u1 u    #d7 a10.3390/molecules220609002DOI1 aOLIVEIRA JÚNIOR, R. G. de  aAntinociceptive effect of the essential oil from Croton conduplicatus Kunth (Euphorbiaceae).h[electronic resource]  c2017  aMedicinal plants have been widely used in the treatment of chronic pain. In this study, we describe the antinociceptive effect of the essential oil from Croton conduplicatus (the EO 25, 50, and 100 mg/kg, i.p.), a medicinal plant native to Brazil. Antinociceptive activity was investigated by measuring the nociception induced by acetic acid, formalin, hot plate and carrageenan. A docking study was performed with the major constituents of the EO (E-caryophyllene, caryophyllene oxide, and camphor). The EO reduced nociceptive behavior at all doses tested in the acetic acid-induced nociception test (p&lt 0.05). The same was observed in both hases (neurogenic and inflammatory) of the formalin test. When the hot-plate test was conducted, the EO (50 mg/kg) extended the latency time after 60 min of treatment. The EO also reduced leukocyte migration at all doses, suggesting that its antinociceptive effect involves both central and peripheral mechanisms. Pretreatment with glibenclamide and atropine reversed the antinociceptive effect of the EO on the formalin test, suggesting the involvement of K ATP channels and muscarinic receptors. The docking study revealed a satisfactory interaction profile between the major components of the EO and the different muscarinic receptor subtypes (M2, M3, and M4). These results corroborate the medicinal use of C. conduplicatus in folk medicine  aMedicinal plants  aCaatinga  aÓleo essencial  aPlanta medicinal  aVegetação Nativa  aCroton conduplicatus  aEssential oil  aPlanta nativa  aQuebra faca1 aFERRAZ, C. A. A.1 aSILVA, J. C.1 aOLIVEIRA, A. P. de1 aDINIZ, T. C.1 aSILVA, M. G. e1 aQUINTANS JÚNIOR, L. J.1 aSOUZA, A. V. de1 aSANTOS, U. S. dos1 aTURATTI, I. C. C.1 aLOPES, N. P.1 aLORENZO, V. P.1 aALMEIDA, J. R. G. da S.  tMoleculesgv. 22, n. 6, p. 1-14, may. 2017.