01916naa a2200313 a 450000100080000000500110000800800410001902400520006010000130011224501020012526000090022752010260023665000140126265000210127665000200129765300110131765300260132865300180135470000250137270000220139770000170141970000170143670000210145370000220147470000140149670000190151070000220152977300510155120646002024-02-09       2016    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.nano.2016.01.0062DOI1 aMUNK, M.  aBiocompatibility assessment of fibrous nanomaterials in mammalian embryos.h[electronic resource]  c2016  aCurrently there is a growing interest in the use of nanotechnology in reproductive medicine and reproductive biology. However, their toxic effects on mammalian embryos remain poorly understood. In this work, we evaluate the biocompatibility of two fibrous nanomaterials (NMs): cotton cellulose nanofibers (CNF) and carboxylated multiwalled carbon nanotubes (MWCNT-COOH), by performing an investigation of the embryonic development, gene expression (biomarkers focused on cell stress, apoptosis and totipotency) and in situ apoptosis in bovine embryos. Exposure to NMs did not interfere in preimplantation development or in the incidence of apoptosis in the bovine embryo, but they did affect the gene expression. The results presented are important for an understanding of the toxicity of cotton CNF and MWCNT-COOH on mammalian embryos. To our knowledge, we report the first evaluation of biocompatibility between these NMs on preimplantation embryos, which may open a new window for reproductive biomedical applications.  aapoptosis  abiocompatibility  agene expression  aFibras  aFibrous nanomaterials  aNanomateriais1 aCAMARGO, L. S. de A.1 aQUINTAO, C. C. R.1 aSILVA, S. R.1 aSOUZA, E. D.1 aRAPOSO, N. R. B.1 aMARCONCINI, J. M.1 aJORIO, A.1 aLADEIRA, L. O.1 aBRANDAO, H. de M.  tNanomedicinegv. 12, n. 5, p. 1151-1159, 2016.