01922naa a2200253 a 450000100080000000500110000800800410001902400260006010000180008624501020010426000090020652012140021565300250142965300100145465300120146465300130147665300250148965300260151470000170154070000190155770000190157670000280159577300450162320614082019-02-21       2017    bl uuuu     u00u1 u    #d7 a10.3390/f80100272DOI1 aSTUEPP, C. A.  aThe use of auxin quantification for understanding clonal tree propagation.h[electronic resource]  c2017  aQualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.  aAdventitious rooting  aAuxin  aCloning  aCuttings  aIndole-3-acetic acid  aIndolyl-3-acetic acid1 aWENDLING, I.1 aTRUEMAN, S. J.1 aKOEHLER, H. S.1 aZUFFELLATO-RIBAS, K. C.  tForestsgv. 5, n. 7, p. 1737-1752, 2017.