02658naa a2200421 a 450000100080000000500110000800800410001902400380006010000240009824501760012226000090029852013680030765000160167565000230169165000200171465000150173465000250174965000250177465000330179965000150183265000190184765000150186665000240188165000120190565000210191765000180193865300210195665300240197770000190200170000220202070000250204270000140206770000210208170000200210270000170212270000280213977300690216720612102020-10-19       2017    bl uuuu     u00u1 u    #d7 adx.doi.org/10.1111/jfd.125092DOI1 aFUKUSHIMA, H. C. S.  aLactococcus garvieae outbreaks in Brazilian farms Lactococcosis in Pseudoplatystoma sp.bdevelopment of an autogenous vaccine as a control strategy.h[electronic resource]  c2017  aAbstract: This study evaluated the control of streptococcosis outbreaks in Brazil, isolated from diseased sorubim and identified as Lactococcus garvieae by genetic sequencing. This report determined the potential for lactococcosis control in sorubim Pseudoplatystoma sp. with two vaccines: an aqueous-based, whole-cell inactivated vaccine (bacterin) and an oil-adjuvanted bacterin. Their efficacy was evaluated at 30 days post-vaccination (d.p.v.) by challenge with L. garvieae, and the antibody production response at 15, 30 and 60 d.p.v. and the non-specific immune response were compared amongst treatments. High protection levels (P &lt 0.05) were achieved with the oil-adjuvanted vaccine with a relative percentage survival value of 81.7% at 30 d.p.v. Additionally, the oil-adjuvanted vaccine increased the immunogenicity of the bacterin as indicated by greater agglutination antibody titres from 15 until 60 d.p.v. This is the first report of a positive effect of vaccine administration on the specific immunity of sorubim, and the study showed that a specific antibody plays an important role in sorubim defence against lactococcosis because the innate immune responses were similar in all of the studied animals. These results demonstrated that oil-adjuvanted vaccine can be an effective alternative for the protection of sorubim from L. garvieae disease.  aAquaculture  aBacterial vaccines  aFreshwater fish  aImmunology  aInactivated vaccines  aLactococcus garvieae  aPseudoplatystoma reticulatum  aBacteriose  aDoença animal  aImunologia  aPeixe de água doce  aSurubim  aVacina inativada  aVacina oleosa  aKoch's postulate  aMolecular diagnosis1 aLEAL, C. A. G.1 aCAVALCANTE, R. B.1 aFIGUEIREDO, H. C. P.1 aARIJO, S.1 aMORIÑIGO, M. A.1 aISHIKAWA, M. M.1 aBORRA, R. C.1 aRANZANI-PAIVA, M. J. T.  tJournal of Fish Diseases, Oxfordgv. 40, n. 2, p. 263-272, 2017.