02194naa a2200301 a 450000100080000000500110000800800410001902200140006002400350007410000310010924500700014026000090021052013940021965000200161365000160163365000110164965300090166070000190166970000160168870000190170470000160172370000250173970000170176470000200178170000230180170000200182477300480184420590492017-06-01       2016    bl uuuu     u00u1 u    #d  a1471-21647 a10.1186/s12864-016-2408-92DOI1 aARDISSON-ARAÚJO, D. M. P.  aA betabaculovirus encoding a gp64 homolog.h[electronic resource]  c2016  aA betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses. DisaGV represents a distinct lineage of the genus Betabaculovirus. It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64, which was reported solely in group 1 alphabaculovirus genomes so far.  aBetabaculovirus  aLepidoptera  aGenoma  aCana1 aPEREIRA, B. T.1 aMELO, F. L.1 aRIBEIRO, B. M.1 aBÁO, S. N.1 aZANOTTO, P. M. de A.1 aMOSCARDI, F.1 aKITAJIMA, E. W.1 aSOSA-GÓMEZ, D. R.1 aWOLFF, J. L. C.  tBMC Genomicsgv. 17, n. 1, 9 p., Feb. 2016.