02352naa a2200169 a 450000100080000000500110000800800410001910000160006024501080007626000090018430000120019350000190020552017140022465000160193870000220195477302060197620536062016-09-28       2016    bl ---       0--  u    #d1 aBINNECK, E.  aThe complete genome of Metarhizium rileyi, a key fungal pathogen of Lepidoptera.h[electronic resource]  c2016  ap. 110.  aPoster: FU-23.  aThe fungus Metarhizium rileyi (= Nomuraea rileyi) is a key regulatory agent of lepidopteran populations. In soybean and cotton agroecosystems, it causes major epizootics decimating important lepidopteran pests such as velvetbean caterpillar, soybean loopers, green cloverworm, cotton leafworm and others. Despite its recent phylogenetic transfer to Metarhizium, M. rileyi displays unusually high specificity by infecting only lepidopteran species. The complete genome of M. rileyi was assembled de novo using short-read Illumina data from paired-end and mate- pair libraries. A total of 311 scaffolds were constructed (&gt 1 kb, N50 = 800 kb), with a total length of 31,007,635 bp. An ab initio annotation, using a set of 2,159 gene stuctures of Metarhizium robertsii ARSEF 23 to train the model, predicted a total of 10,880 genes coding for proteins in this genome. Orthologous genes were detected in M. robertsii and M. acridum: 4,806 orthologs in both species, 349 orthologs only in M. robertsii, and 301 orthologs only in M. acridum. In-paralogs were not counted. Divergent genes (4,534) with no shared orthologs were compared to annotation data for known functions and were categorized according to an enrichment analysis of function-related aspects. A remarkable and more numerous category of genes appears to be polyketide synthases, possibly involved in the secondary metabolism required for virulence to insect hosts. This study provides the genome sequence and annotation of M. rileyi. Comparative studies of its genome among different isolates and species could provide new insights on differential pathogenicity and improved understandings of its relationships to phylogenetically related species.  aEntomologia1 aSOSA-GOMEZ, D. R.  tIn: ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 49.; INTERNATIONAL CONGRESS ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 2016. Tours. [S.l.]: Society for Invertebrate Pathology, 2016.