01654naa a2200301 a 450000100080000000500110000800800410001910000250006024501210008526000090020650000240021552006680023965000150090765000190092265000190094165300450096065300140100565300160101965300400103565300360107565300300111165300460114170000180118770000190120570000160122470000220124077300900126220505542017-03-07       2016    bl uuuu     u00u1 u    #d1 aGONÇALVES, A. N. D.  aGenotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.h[electronic resource]  c2016  aNotas científicas.  aThe objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.  aGenotyping  aPrion diseases  aPeste de cocar  aEncefalopatia espongiforme transmissivel  aGene PRNP  aGenotipagem  aPolimorfismo de nucleotídeo único  aSingle nucleotide polymorphisms  aSpongiform encephalopathy  aTransmissible spongiform encephalopathies1 aSOARES, C. O.1 aSANCHES, S. C.1 aREIS, F. A.1 aROSINHA, G. M. S.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 51, n. 5, p.684-687, maio, 2016.