02036naa a2200277 a 450000100080000000500110000800800410001910000220006024501520008226000090023452011370024365000160138065000210139665000230141765300300144065300250147065300110149565300300150665300220153665300240155865300240158270000190160670000230162570000190164877300910166720499572016-08-01       2016    bl uuuu     u00u1 u    #d1 aFONSECA, E. T. da  aCulture, characterization and differentiation of neural precursors from the central nervous system of guinea pigs (Cavia porcellus Linnaeus, 1758).  c2016  aPotentially neurogenic areas were initially identified by incorporation of bromodeoxyuridine (BrdU) in cells underlying the subventricular zone (SVZ) of the lateral ventricles wall, hippocampus and olfactory bulbs of newborn guinea pigs. Neural precursors from the SVZ were cultured in suspension, generating neurospheres (NSFs), which, upon dissociation were able to generate new NSFs. Upon culture in the absence of growth factors, cells dissociated from NSFs displayed evidence for neural differentiation, giving rise to cells from neural lineage. Flow cytometry analysis for of NSFs-derived cells after differentiation revealed approximately 13.3% nestin positive, 5.5% Beta-III-tubulin positive, 9% GFAP positive and 7.8% mGalC positive. Functional assays by measurement of calcium influx upon gamma butiric amino acid (GABA) and glutamate stimuli, revealed stimulation in differentiated cells, an indicator of neuronal differentiation. The ability of guinea pig SVZ cells to originate functional neurons in vitro is promising for research and towards a future use of neural stem cells in the therapy of neurological disorders.  aGuinea pigs  aIn vitro culture  aProdução vegetal  aCaracterização in vitro  aCelula-tronco neural  aCobaio  aIn vitro characterization  aNeural stem cells  aSubventricular zone  aZona subventricular1 aGALINDO, L. T.1 aPORCIONATTO, M. A.1 aMIGLINO, M. A.  tPesquisa Veterinária Brasileira, Brasília, DFgv. 36 (supl. 1), p. 69-78, jun. 2016.