02737naa a2200349 a 450000100080000000500110000800800410001902400350006010000200009524501440011526000090025950000350026852016730030365000150197665000150199165000280200665000210203465000170205565000230207265000190209565000150211465000100212965000170213965000120215665300240216865300220219270000190221470000170223370000180225070000270226877300920229520458202017-02-21       2016    bl uuuu     u00u1 u    #d7 a10.1007/s00253-016-7597-22DOI1 aMASCARIN, G. M.  aImproved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes.h[electronic resource]  c2016  aPublished online: 20 May 2016.  aThe yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to &lt5 % water content equivalent to aw &lt 0.3. The viability of B. bassiana blastospores after air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 °C compared with 28 °C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 °C, viability was significantly prolonged for both air- and spraydried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (∼twofold higher half-life) when stored at 28 °C. After storage for 4 months at 28 °C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low aw and oxygen availability) that improved blastospore storage stability at 28 °C and will facilitate the commercial development of blastospores-based bioinsecticides.  aAir drying  aDesiccants  aFree radical scavengers  aMycoinsecticides  aSpray drying  aBeauveria bassiana  aBemisia tabaci  aDessecante  aFungo  aMosca branca  aSecagem  aLiquid fermentation  aStorage stability1 aJACKSON, M. A.1 aBEHLE, R. W.1 aKOBORI, N. N.1 aDELALIBERA JÚNIOR, I.  tApplied Microbiology and Biotechnology, Berlingv. 100, n. 19, p. 8359-8370, Oct. 2016.