02131naa a2200301 a 450000100080000000500110000800800410001910000180006024501210007826000090019952012640020865000190147265000180149165000230150965300200153265300120155265300120156465300130157665300200158965300140160965300240162370000190164770000190166670000170168570000150170270000220171777300900173920442442016-04-29       2015    bl uuuu     u00u1 u    #d1 aSPRADA, A. G.  aToxicity and oxidative stress of canine mesanchymal stromal cells from adipose tissue in different culture passages.  c2015  aStem cells in regenerative therapy have received attention from researchers in recent decades. The culture of these cells allows studies about their behavior and metabolism. Thus, cell culture is the basis for cell therapy and tissue engineering researches. A major concern regarding the use of cultivated stem cell in human or veterinary clinical routine is the risk of carcinogenesis. Cellular activities require a balanced redox state. However, when there is an imbalance in this state, oxidative stress occurs. Oxidative stress contributes to cytotoxicity, which may result in cell death or genomic alterations, favoring the development of cancer cells. The aim of this study was to determine whether there are differences in the behavior of cultured mesenchymal stem cells from canine adipose tissue according to its site of collection (omentum and subcutaneous) evaluating the rate of proliferation, viability, level of oxidative stress and cytotoxicity over six passages. For this experiment, two samples of adipose tissue from subcutaneous and omentum where taken from a female dog corpse, 13 years old, Pitbull. The results showed greater levels of oxidative stress in the first and last passages of both groups, favoring cytotoxicity and cell death.  aCell viability  aFree radicals  aProdução vegetal  aCélulas-tronco  aCulture  aPassage  aPassagem  aRadicais livres  aStem cell  aViabilidade celular1 aROSA, M. P. da1 aMACHADO, A. K.1 aPIPPI, N. L.1 aBAYARD, P.1 aCRUZ, I. B. M. da  tPesquisa Veterinária Brasileira, Brasília, DFgv. 35, supl. 1, p. 15-20, dez. 2015.