02088naa a2200301 a 450000100080000000500110000800800410001910000200006024500940008026000090017452012310018365000260141465000120144065000150145265000210146765000130148865300090150165300170151065300320152770000210155970000200158070000180160070000150161870000220163370000200165570000210167577300900169620437982016-04-22       2015    bl uuuu     u00u1 u    #d1 aGRESSLER, L. T.  aBiofilm formation by Rhodococcus equi and putative association with macrolide resistance.  c2015  aRhodococcus equi is a facultative intracellular pathogen, which cause severe pyogranulomatous pneumonia in foals and tuberculosis-like lesions in humans. Its ability to form biofilm was described in strains isolated from chronic diseases associated to treatment failures in humans. This study aimed to verify the biofilm formation by 113 R. equi isolated from equine samples (clinical and fecal) using two different methods (biofilm--culturing with and without additional glucose and epifluorescence microscopy). We also aimed to determine the efficacy of azithromycin, clarithromycin and erythromycin on R. equi in established biofilm. We found 80.5% (26/41) and 63% (58/72) biofilm--positive isolates, in fecal and clinical samples, respectively. The additional glucose increased the biofilm formation by R. equi fecal samples, but not by clinical samples. The antimicrobials tested herein were not able to eradicate R. equi in biofilm even at higher concentrations. This is the first study showing the biofilm formation by R. equi isolated from equine samples. Our findings indicate that R. equi biofilm-producers may be more resistant to the antimicrobials evaluated. Further studies are warranted to test this hypothesis.  aAntibiotic resistance  aBiofilm  aMacrolides  aRhodococcus equi  aBiofilme  aDAPI  aMacrolídeos  aResistência antimicrobiana1 aVARGAS, A. C. de1 aCOSTA, M. M. da1 aSUTILI, F. J.1 aSCHWAB, M.1 aPEREIRA, D. I. B.1 aSANGIONI, L. A.1 aBOTTON, S. de A.  tPesquisa Veterinária Brasileira, Brasília, DFgv. 35, n. 10, p. 835-841, out. 2015.