01888naa a2200253 a 450000100080000000500110000800800410001902200140006002400350007410000200010924501660012926000090029552010770030465000120138165000230139365300230141665300340143965300170147370000230149070000220151370000200153570000220155577300570157720434802016-04-15       2016    bl uuuu     u00u1 u    #d  a1756-46467 a10.1016/j.jff.2016.03.0272DOI1 aALVES, N. E. G.  aDigested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages.h[electronic resource]  c2016  aOxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in humanTHP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin?pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-α (TNF-α) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1.  aFeijão  aPhaseolus vulgaris  aBioactive peptides  aLectin type ox-LDL receptor 1  aOxidized LDL1 aVASCONCELOS, C. M.1 aBASSINELLO, P. Z.1 aMEJIA, E. G. de1 aMARTINO, H. S. D.  tJournal of Functional Foodsgv. 24, p. 97-111, 2016.