01675nam a2200181 a 450000100080000000500110000800800410001910000210006024501640008126001390024552009970038465300110138170000230139270000200141570000200143570000190145570000190147420417482024-04-25       2015    bl uuuu     u00u1 u    #d1 aAQUINO, S. O. de  aFunctional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum.h[electronic resource]  aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-46.c2015  aIn many higher plants, DREB genes were shown to be involved in the transduction pathways of abiotic stress. Previous results showed that CcDREB1D gene expression increased under drought stress in leaves of drought-tolerant clone 14 of Coffea canephora Conilon but not in those of the drought-susceptible clone 22. By sequencing the DREB1D promoter regions of these two clones, several nucleic polymorphisms (?single nucleotide polymorphism? [SNP] and insertion/deletion [INDELs]) were found. In order to know if these polymorphisms could explain the differences of DREB1D gene expression observed between the clones 14 and 22, 5' deletions of several alleles of the CcDREB1D promoter regions were made and cloned independently into the binary vector pBI101 in order to analyze their ability to control the expression of the uidA reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This work shows preliminary results of DREB1D promoter constructs analyzed in leaves of N. tabacum.  aCoffee1 aCARNEIRO, F. de A.1 aRÊGO, E. C. S.1 aALVES, G. S. C.1 aMARRACCINI. P.1 aANDRADE, A. C.