01309naa a2200205 a 450000100080000000500110000800800410001910000220006024501060008226000090018852007140019765000080091165000160091965000230093565000200095865300190097865300220099770000190101977300650103820415812016-03-22       2015    bl uuuu     u00u1 u    #d1 aALMEIDA, R. P. de  aITS-2 sequences-based identification of Trichogramma species in South America.h[electronic resource]  c2015  aITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.  aDNA  aHimenoptero  aMarcador molecular  aTrichogramma sp  aEgg parasitoid  aMolecular markers1 aSTOUTHAMER, R.  tBrazilian Journal of Biologygv. 75, n. 4, p. 974-982, 2015.