01549naa a2200229 a 450000100080000000500110000800800410001910000220006024501240008226000090020630000140021552008080022965000230103765300330106070000130109370000230110670000210112970000200115070000160117070000250118677301080121120379942023-06-27       2016    bl uuuu     u00u1 u    #d1 aBRANDAO, H. de M.  aCarbon nanotubes as a DNA delivery agent for generation of genetically modified mammals embryos.h[electronic resource]  c2016  ap. 31-56.  aConteúdo: 1) Introduction; 1.1) Oocyte and Embryo Structures; 1.2) Applications of Genetically Modified Animals (GMA); 2) Conventional Gene Transfer Methods for Generating Transgenic Animals; 2.1) Pronuclear Microinfection; 2.2) Embryonic Stem Cell Transgenesis; 2.3) Sperm-Mediated Gene Transfer (SMGT); 2.4) Retroviral Vectors; 2.5) Somatic Cell Nuclear Transfer (SCNT); 2.6) Zinc-Finger Nucleases (ZFN) and Transcription Activator-like Effector Nucleases (TALENs); 3) Carbon Nanotubes (CNT) as a Gene Transfer Agent; 3.1) Interaction of CNT and DNA; 3.2) Contribuition of CNTs to Convencional Techniques; 3.3) Potential DNA Delivery to Embryo Without Micromanipulation; 4) Genotoxicity; 4.1) General Aspects; 4.2) Detection Techniques, Toxicity and Genotoxicity of CNTs; 5) Summary and Perspectives;  aTransgenic Animals  aGenetically modified animals1 aMUNK, M.1 aCARVALHO, B. C. de1 aGUIMARAES, A. S.1 aCARVALHO, W. A.1 aGERN, J. C.1 aCAMARGO, L. S. de A.  tIn: JORIO, A. (ed.). Bioengineering applications of carbon nanostructures. Switzerland: Springer, 2016.