01624naa a2200217 a 450000100080000000500110000800800410001910000200006024501630008026000090024352009360025265000180118865300120120670000190121870000200123770000220125770000220127970000190130170000180132077300680133820377512024-02-03       2015    bl uuuu     u00u1 u    #d1 aSILVA, A. P. C.  aProtection Provided by an Encapsulated Live Attenuated ?abcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model.h[electronic resource]  c2015  aThis study aimed to evaluate the Brucella ovis ΔabcBA strain as a vaccine candidate in the murine model. BALB/c mice were subcutaneously or intraperitoneally immunized with a single dose or three doses of the B. ovis ΔabcBA strain and then were challenged with wild-type B. ovis. Single or multiple immunizations provided only mild protection, with significantly smaller numbers of wild-type B. ovis CFU in the livers of immunized mice but not in the spleens. Encapsulation of B. ovis ΔabcBA significantly improved protection against experimental challenges in both BALB/c and C57BL/6 mice. Furthermore, immunization with encapsulated B. ovis ΔabcBA markedly prevented lesions in the spleens and livers of experimentally challenged mice. These results demonstrated that the encapsulated B. ovis ΔabcBA strain confers protection to mice; therefore, this strain has potential as a vaccine candidate for rams.  aanimal health  aVaccine1 aMACÊDO, A. A.1 aSILVA, T. M. A.1 aXIMENES, L. C. A.1 aBRANDAO, H. de M.1 aPAIXÃO, T. A.1 aSANTOS, R. L.  tClinical and Vaccine Immunologygv. 22, n. 7, p. 789-973, 2015.