02109naa a2200229 a 450000100080000000500110000800800410001910000240006024502880008426000090037252012420038165000290162365300080165265300180166065300270167870000260170570000220173170000160175370000160176970000260178577300680181120358202024-02-01       2015    bl uuuu     u00u1 u    #d1 aCASTRO, R. de J. C.  aA Rapid Method for Determination of the Main Conjugated Linoleic Acid Precursors (C18b2 n-6 and C18:3 n-3) in Forage by Capillary Zone Electrophoresis with Ultraviolet Detection Using Gas Chromatography with Flame Ionization Detection as a Comparative Method.h[electronic resource]  c2015  aAbstract: A rapid method has been proposed for determination of the main conjugated linoleic acid precursors such as linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acids in forages by capillary zone electrophoresis (CZE) with direct UV detection at 200 nm. Among the fatty acids found in forages, C18:2 n-6 and C18:3 n-3 have received particular attention due to their roles as precursors for the synthesis of conjugated linoleic acid, a class of health-enhancing compounds that is predominantly found in dairy products. The electrolyte background consisted of 12.0 mmol/L tetraborate buffer (pH 9.2) added to 12.0 mmol/L Brij 35(®), 17% acetonitrile, and 33% methanol. Under the optimized conditions, the baseline separation of C18:2 n-6 and C18:3 n-3 was achieved within 4 min. The CZE-UV method was compared to GC with a flame ionization detector, which is the American Oil Chemists' Society (AOCS 996.06) official method for fatty acid analysis. The methods did not show any evidence of significant differences within 95% confidence interval (P &gt 0.05). The CZE-UV method was successfully applied to the analysis of 80 genotypes of Brachiaria ruzizienses clones submitted to a genetic improvement program in agricultural research.  aconjugated linoleic acid  aCLA  aCZE-UV method  aFatty acids in forages1 aSOUZA SOBRINHO, F. de1 aGAMA, M. A. S. da1 aSATO, R. T.1 aLIMA, L. L.1 aOLIVEIRA, M. A. L. de  tJournal of AOAC Internationalgv. 98, n. 6, p. 1591-1597, 2015.